Herbal formula BaWeiBaiDuSan alleviates polymicrobial sepsis-induced liver injury via increasing the gut microbiota Lactobacillus johnsonii and regulating macrophage anti-inflammatory activity in mice

• DOI: 10.1016/j.apsb.2022.10.016
• Publication Date: 2022-10-22T16:23:00Z
• Authors: Xiaoqing Fan; Chutian Mai; Ling Zuo; Jumin Huang; Chun Xie; Zebo Jiang; Runze Li; Xiaojun Yao; Xingxing Fan; Qibiao Wu; Peiyu Yan; Liang Liu; Jianxin Chen; Ying Xie; Elaine Lai-Han Leung
• Keywords: 0301 basic medicine; 03 medical and health sciences; Sepsis-induced liver injury; Macrophages; Original Article; Therapeutics. Pharmacology; RM1-950; 16S PacBio SMRT sequencing; BaWeiBaiDuSan; Network pharmacology; Lactobacillus johnsonii

Sepsis-induced liver injury (SILI) is an important cause of septicemia deaths. BaWeiBaiDuSan (BWBDS) was extracted from a formula Panax ginseng C. A. Meyer, Lilium brownie F. E. Brown ex Miellez var. viridulum Baker, Polygonatum sibiricum Delar. Redoute, Lonicera japonica Thunb., Hippophae rhamnoides Linn., Amygdalus Communis Vas, Platycodon grandiflorus (Jacq.) DC., and Cortex Phelloderdri. Herein, we investigated whether the BWBDS treatment could reverse SILI by mechanism modulating gut microbiota. protected mice against SILI, which associated with promoting macrophage anti-inflammatory activity enhancing intestinal integrity. selectively promoted growth Lactobacillus johnsonii (L. johnsonii) in cecal ligation puncture treated mice. Fecal microbiota transplantation indicated that bacteria correlated sepsis required for anti-sepsis effects. Notably, L. significantly reduced activity, increasing interleukin-10+ M2 production Furthermore, heat inactivation (HI-L. alleviated SILI. Our findings revealed as novel prebiotic probiotic may be used to treat The potential underlying at least part, via johnsonii-dependent immune regulation production.