Glycodiversification of gentamicins through in vivo glycosyltransferase swapping enabled the creation of novel hybrid aminoglycoside antibiotics with potent activity and low ototoxicity

• DOI: 10.1016/j.apsb.2024.04.030
• Publication Date: 2024-05-03T03:39:38Z
• Authors: Xinyun Jian; Cheng Wang; Shijuan Wu; Guo Sun; Chuan Huang; Chengbing Qiu; Yuanzheng Liu; Peter F. Leadlay; Dong Liu; Zixin Deng; Fuling Zhou; Yuhui Sun
• Keywords: Ototoxicity; Broad spectrum; Nephrotoxicity

Aminoglycosides (AGs) are a class of antibiotics with broad spectrum activity. However, their use is limited by safety concerns associated nephrotoxicity and ototoxicity, as well drug resistance. To address these issues, semi-synthetic approaches for modifying natural AGs have generated new generations AGs, however, types modification due to significant challenges in synthesis. This study explores novel approach that harness the bacterial biosynthetic machinery gentamicins kanamycins create hybrid AGs. was achieved glycodiversification