Co-occurrence network of microbes linking growth and immunity parameters with the gut microbiota in Nile tilapia (Oreochromis niloticus) after feeding with fermented soybean meal

• DOI: 10.1016/j.aqrep.2022.101280
• Publication Date: 2022-08-06T18:11:52Z
• Authors: Lu Li; Jingjing Song; Chunyan Peng; Zicong Yang; Lin Wang; Juncheng Lin; Liangbo Li; Zhicheng Huang; Bin Gong
• Keywords: Nile tilapia; Bacillus coagulans; Lactobacillus fermentum

The supplementation of fish feed with fermented soybean meal (FSBM) as an additive is widely used to produce aquatic feed. However, the mechanism by which FSBM regulates gut microbes in are still largely unknown. In present work, Lactobacillus fermentum (Lactobacillus group), Bacillus natto (Bacillus and Saccharomyces cerevisiae (Saccharomyces group) was added diets, effects these diets on microbiota, growth performance [Weight gain rate (WGR) Specific (SGR)], immunity [superoxide dismutase (SOD), lysozyme (LSZ) alkaline phosphatase (AKP)] Nile tilapia assessed. Compared Nontreated group, remarkably improved WGR, SGR, AKP, LSZ (p < 0.05). SOD activity significantly lower group than other groups High-throughput sequencing V3-V4 region 16S rRNA gene analyze microbiota tilapia. PCoA UPGMA showed that similarity between higher. increased Chao1 index number observed species 0.05), while Simpson Shannon indices 0.01). Corynebacterium Mycobacterium were enriched groups, Aquabacterium Acidovorax group. LEfSe analysis Rhizobiales, Thermomicrobia, Myxococcales Gemmatales biomarker Mycobacterium, Clavibacter Coxiellaceae Brevundimonas More interestingly, co-occurrence network indicated supplemented L. or S. more likely impact some played pivotal roles network. fermentum- cerevisiae- SBM tended activate similar "big team" promote their cooperation perform certain functions. This research fermentum, B. natto, exerted different effect growth, immunity, tilapia, could partially explain relationship immune parameters microbiomic data.