Gut microbiota changes in Hexagrammos otakii during development and association with diets based on metagenomics sequencing

• DOI: 10.1016/j.aqrep.2023.101881
• Publication Date: 2023-12-11T22:28:02Z
• Authors: Ying Fan; Youhong Wang; Li Li; Xiaolu Wang; Xiaoqing Yu; La Xu; Shuxian Wang; Fawen Hu; Chunlei Gai; Jing Diao; Haibin Ye; Fusheng Hou; Xintian Liu
• Keywords: Lactococcus; Microbial Metabolism

The development of microbial communities is important for the growth fat greenling Hexagrammos otakii; however, relationship between and fish diet poorly understood. Here, we used metagenomics sequencing to investigate gut microbiota H. otakii at three key time points during development. diversity community composition presented stage-specific signatures appeared shift with different diets environmental conditions such as NH4 + -NO2- etc. Metagenomics analysis indicated that dominant phyla were Proteobacteria Firmicutes. With dietary changes from rotifers Artemia formulated feed, genera shifted Vibrio Photobacterium Lactococcus, variations in increased age. Based on functional annotation using Kyoto Encyclopedia Genes Genomes database, stages affected metabolic function microbiome by activating carbohydrate amino acid metabolism while weakening lipid nucleotide metabolism. In addition, predicted Carbohydrate-Active enZymes glycoside hydrolase glycosyl transferases may be involved food degradation. Our results suggest assembly was coupled animal host age-associated biotic features changes. Diet had a considerable effect these distinct times, indicating rapidly adapted shifts. therefore likely plays an indispensable role nutrient turnover fermentation. These findings can provide guidance probiotics application feed additives.