Mitochondrial bioenergetic dysfunction linked to myxomatous mitral valve degeneration explored by PBMCs metabolism analysis

• DOI: 10.1016/j.bbabio.2024.149505
• Publication Date: 2024-08-16T22:59:04Z
• Authors: Silvia Granata; Chiara Bernardini; Patrycja Anna Glo...; Giovanni Romito; Roberta Salaroli; Cristina Algieri; Antonia Cugliari; Micaela Fabbri; Fabiana Trombetti; Augusta Zannoni; Salvatore Nesci

Impaired mitochondria cause an impressive decrease in ATP production becoming a common condition of cardiovascular diseases. Myxomatous mitral valve disease (MMVD) is characterized by mitochondrial dysfunction. By a non-invasive procedure of metabolism analysis on peripheral blood mononuclear cells, we exploit ex-vivo studies that directly constitute a translational approach to evaluate the cell bioenergetics. Cell ATP production decreased in the presence of MMVD, whereas glycolysis was unaffected. In MMVD, the mitochondrial activity underwent a significant reduction of basal respiration, maximal respiration, and ATP production. Our results depicted a pathological condition of MMVD characterized by cell metabolism deprived of mitochondrial energy support.