Single-cell analysis of dorsal root ganglia reveals metalloproteinase signaling in satellite glial cells and pain

• DOI: 10.1016/j.bbi.2023.08.005
• Publication Date: 2023-08-07T15:34:37Z
• Authors: Raquel Tonello; Arthur Silveira P...; Sang Hoon Lee; Cinder Faith Cohen; Wenrui Xie; Aditi Paranjpe; Jueun Roh; Chul-Kyu Park; Gehoon Chung; Judith A. Strong; Jun-Ming Zhang; Temugin Berta
• Keywords: Sensory Receptor Cells; Ganglia, Spinal; Humans; Pain; Single-Cell Analysis; Neuroglia; Signal Transduction

Satellite glial cells (SGCs) are among the most abundant non-neuronal in dorsal root ganglia (DRGs) and closely envelop sensory neurons that detect painful stimuli. However, little is still known about their homeostatic activities contribution to pain. Using single-cell RNA sequencing (scRNA-seq), we were able obtain a unique transcriptional profile for SGCs. We found enriched expression of tissue inhibitor metalloproteinase 3 (TIMP3) other metalloproteinases Small interfering neutralizing antibody experiments revealed TIMP3 modulates somatosensory decreased after paclitaxel treatment, its rescue by delivery recombinant protein reversed prevented paclitaxel-induced also established directly impacts signaling cultured SGCs, which may be used identify potential new treatments Therefore, our results reveal pathway SGCs proper processing stimuli discovery novel pain treatments.