Cell cycle-dependent regulation of Aurora kinase B mRNA by the Microprocessor complex

• DOI: 10.1016/j.bbrc.2014.02.104
• Publication Date: 2014-03-01T00:50:08Z
• Authors: Eunsun Jung; Youngmo Seong; Jae Hong Seo; Young-Soo Kwon; Hoseok Song
• Keywords: Ribonuclease III; 0301 basic medicine; Base Sequence; RNA Stability; Proteins; RNA-Binding Proteins; Cell Cycle Checkpoints; Models, Biological; DEAD-box RNA Helicases; Mice; 03 medical and health sciences; Gene Knockdown Techniques; Multiprotein Complexes; Mutation; Animals; Aurora Kinase B; Humans; Nucleic Acid Conformation; RNA, Messenger; Cells, Cultured; HeLa Cells

Aurora kinase B regulates the segregation of chromosomes and the spindle checkpoint during mitosis. In this study, we showed that the Microprocessor complex, which is responsible for the processing of the primary transcripts during the generation of microRNAs, destabilizes the mRNA of Aurora kinase B in human cells. The Microprocessor-mediated cleavage kept Aurora kinase B at a low level and prevented premature entrance into mitosis. The cleavage was reduced during mitosis leading to the accumulation of Aurora kinase B mRNA and protein. In addition to Aurora kinase B mRNA, the processing of other primary transcripts of miRNAs were also decreased during mitosis. We found that the cleavage was dependent on an RNA helicase, DDX5, and the association of DDX5 and DDX17 with the Microprocessor was reduced during mitosis. Thus, we propose a novel mechanism by which the Microprocessor complex regulates stability of Aurora kinase B mRNA and cell cycle progression.