Toward understanding barnacle cementing by characterization of one cement protein-100kDa in Amphibalanus amphitrite

• DOI: 10.1016/j.bbrc.2017.11.101
• Publication Date: 2017-11-20T03:33:55Z
• Authors: Li-Sheng He; Gen Zhang; Yong Wang; Guo-Yong Yan; Pei-Yuan Qian
• Keywords: Barnacle; 0301 basic medicine; Settlement; Life Cycle Stages; Evolution; Molecular Sequence Data; Thoracica; Arthropod Proteins; 03 medical and health sciences; Cement protein; Organ Specificity; Adhesives; Animals; Tissue Distribution; Amino Acid Sequence; Cp100k

Barnacles, as major fouling organisms, have attracted more attentions. It is no doubt that the study on cement proteins is required to illustrate the mechanism of barnacle cementing. A cement protein defined as Aa-cp100k was characterized from Amphibalanus amphitrite in this study. The amino acid sequence of Aa-cp100k was shown a high similarity to other three barnacles including Megabalanus rosa (Mr-cp100k), Tetraclita japonica formosana (Tj-cp100k) and Pollicipes pollicipes (Pp-cp100k). Moreover, the localization of Aa-cp100k in the vacuoles of cyprid β secretory cells and the adult cement gland cells by immunofluorescence microscopy, indicating that Aa-cp100k existed in both cyprid and adult barnacle. Aa-cp100k from basal plate could be dissolved in urea buffer without high concentration of dithiothreitol (DTT), different from that in Megabalanus rosa, implying diverse possible roles of cp100k in cementing.