The role of calcium-binding protein S100g (CalbindinD-9K) and annexin A10 in acute pancreatitis

• DOI: 10.1016/j.bbrc.2020.03.155
• Publication Date: 2020-04-02T21:14:47Z
• Authors: Hirosato Mashima; Kenichi Takahashi; Masanari Sekine; Satohiro Matsumoto; Takeharu Asano; Takeshi Uehara; Junichi Fujiwara; Haruka Otake; Takehiro Ishii; Shuhei Yoshikawa; Takaya Miura; Yudai Koito; Hitomi Kashima; Keita Matsumoto; Hirohide Ohnishi
• Keywords: Acinar cell; Calcium in biology; Viability assay

We reported that the pancreas of interferon-regulatory factor (IRF) 2 knock-out (KO) mouse represents an early phase acute pancreatitis, including defective regulatory exocytosis, intracellular activation trypsin, and disturbance autophagy. The significantly upregulated downregulated genes in IRF2 KO have been reported. catalogue gene transcripts included two types calcium-binding proteins (S100 calcium binding protein G [S100g] Annexin A10 [Anxa10]), which were highly pancreas. As signal plays a pivotal role exocytosis its is related to we then evaluated S100g Anxa10 pancreatitis.We induced cerulein-pancreatitis wild-type mice examined changes expression these by qPCR immunohistochemistry. constructed S100g-overexpressing or Anxa10-overexpressing AR42J cells (AR42J-S100g, AR42J-Anxa10). amylase secretion, ([Ca2+]i), cell viability cells, when incubated with cholecystokinin (CCK).The was increased cerulean-induced pancreatitis. acini patchily stained for cytosol evenly but weakly Anxa10. Stimulation 100pM CCK-8, decreased secretion inhibited [Ca2+]i increase AR42J-S100g cells. These effects weak AR42J-Anxa10 Cell not changed incubation cerulein.In cerulean acini. may work as Ca2+ buffer