Essential amino acid starvation induces cell cycle arrest, autophagy, and inhibits osteogenic differentiation in murine osteoblast

• DOI: 10.1016/j.bbrc.2023.06.055
• Publication Date: 2023-06-17T01:04:13Z
• Authors: Runbo Li; Hirohito Kato; Takaya Nakata; Isao Yamawaki; Nobuhiro Yamauchi; Kazutaka Imai; Yoichiro Taguchi; Makoto Umeda
• Keywords: 2. Zero hunger; Mice; Osteoblasts; Osteogenesis; Autophagy; Animals; Cell Differentiation; Amino Acids, Essential; Cell Cycle Checkpoints

This study investigates the effects of essential amino acid (EAA) starvation on murine osteoblasts cells and the underlying mechanisms. We performed and observed the cell proliferation, autophagy, and osteogenic differentiation under deprivation of EAA in vitro. The results showed that EAA starvation resulted in cell cycle arrest via phosphorylation of the MAPK signaling pathway, leading to inhibition of cell proliferation and osteogenic differentiation. Additionally, the LKB1-AMPK signaling pathway was also found to be phosphorylated, inducing autophagy. These findings highlight the significant role of EAA in regulating cellular processes. Furthermore, this study contributes to our understanding of the effects of nutrient deprivation on cellular physiology and may aid in the development of novel therapeutic strategies for diseases associated with amino acid metabolism.