Long-term survival of transplanted induced pluripotent stem cell-derived neurospheres with nerve conduit into sciatic nerve defects in immunosuppressed mice

• DOI: 10.1016/j.bbrep.2021.100979
• Publication Date: 2021-03-24T07:22:29Z
• Authors: Takuya Yokoi; Takuya Uemura; Kiyohito Takamatsu; Ema Onode; Kosuke Shintani; Shunpei Hama; Yusuke Miyashima; Mitsuhiro Okada; Hiroaki Nakamura
• Keywords: Neurosphere; Nerve guidance conduit; Regenerative Medicine; Peripheral nerve injury; Sciatic nerve injury

Since the advent of induced pluripotent stem cells (iPSCs), clinical trials using iPSC-based cell transplantation therapy have been performed in various fields regenerative medicine. We previously demonstrated that mouse iPSC-derived neurospheres containing neural stem/progenitor with bioabsorbable nerve conduits promoted regeneration long term murine sciatic defect models. However, it remains unclear how grafted survived and worked after implantation. In this study, long-term survival transplanted was evaluated high-immunosuppressed or non-immunosuppressed mice vivo imaging for development peripheral injury. Complete 5-mm defects were created nerves immunosuppressed reconstructed coated labeled ffLuc. The on monitored imaging. 365 days allograft models, but only at least 14 This is first study to find longest rate into demonstrates differences graft between model immune responsive model. future, if are successfully iPSC stock without eliciting an response, axonal will be due longstanding supportive effect direct remyelination and/or secretion trophic factors.