Conditionally immortalised equine skeletal muscle cell lines for in vitro analysis

• DOI: 10.1016/j.bbrep.2022.101391
• Publication Date: 2022-12-05T13:24:24Z
• Authors: Mary F. Rooney; Nuno G.B. Neto; Michael G. Monaghan; Emmeline W. Hill; Richard K. Porter
• Keywords: 0301 basic medicine; Immortalised cells; Equine; QH301-705.5; Skeletal muscle; QD415-436; Bioenergetics; Biochemistry; 03 medical and health sciences; Coenzyme Q10; Cell culture; Biology (General); Research Article

Thoroughbred racehorse performance is largely influenced by a major quantitative trait locus at the myostatin (MSTN) gene which determines aptitude for certain race distances due to promoter region insertion mutation influencing functional phenotypes in skeletal muscle. To develop an vitro system experiments we established three novel equine muscle cell lines reflecting variation phenotype associated with MSTN genotype (CC/II, CT/IN and TT/NN SNP g.66493737C > T/SINE 227 bp polymorphism). Primary myoblasts, isolated from horse gluteus medius, were conditionally immortalised evaluated determine whether metabolic function comparable characteristics previously reported ex vivo horses each genotype. myoblasts temperature sensitive SV40TtsA58 lentivirus vector successfully proliferated could revert their primary differentiate into multinucleated myotubes. Skeletal fibre type, expression, mitochondrial abundance, of lines, consistent equivalent characterisation samples these genotypes. Furthermore, addition coenzyme Q10 (CoQ10) improved function, observation genotypes following supplementation CoQ10 diet. The that phenotypic cells are indicates this will enable efficient cost-effective analyses range different applications including understanding testing nutritional supplements, drug test development doping development. In multi-billion-euro international industry research advances biological likely have considerable impact. genotype-specific may be adapted applied human biomedicine improve effects physiology medicine.