Combinative workflow for mRNA vaccine development

• DOI: 10.1016/j.bbrep.2024.101766
• Publication Date: 2024-06-28T02:27:15Z
• Authors: Renuka Khanzode; Garima Soni; Shalini Srivastava; Sharad Pawar; Rucha Wadapurkar; Ajay Singh
• Keywords: Cloning (programming); spike protein

Recently, mRNA has gained a lot of attention in the field vaccines, gene therapy, and protein replacement therapies. Herein, we are demonstrating comprehensive approach to designing, cloning, characterizing an antigenic cassette for development vaccine COVID-19. The encoding spike SARS-CoV-2 Omicron variant (B.1.1.529) was designed using databases, characterized by