Phaeodactylum tricornutum as a potential feedstock for an integrated biorefinery process under varying cultivation conditions

• DOI: 10.1016/j.bcab.2022.102508
• Publication Date: 2022-10-06T06:11:10Z
• Authors: Duygu Ova Ozcan; Bikem Ovez
• Keywords: Phenol; Extraction; Lipid; Phaeodactylum tricornutum; Oil; 01 natural sciences; 6. Clean water; And transesterification; Microalgae; Biodiesel; Antioxidant; 0105 earth and related environmental sciences

TUBITAK; EBILTEM as Project; Ege University Office of Scientific Research Projects; [1002]; [213M623]; [BIDEB 2211/A]; [2015/BIL/016]; [12-MUH-031]; [13-MUH-032]; [15-MUH-034]; [17-MUH-043]<br/>In this study, the effects of temperature, light intensity, and aeration rate on the biomass, total lipid, and the residual water-soluble fractions of Phaeodactylum tricornutum were ascertained. The maximum total lipid yield of 22.39% was attained at a n-hexane/biomass ratio of 200/1, Soxhlet extraction time of 6 h and a heating rate level of 6 at 69 degrees C. The optimum fatty acid composition with a mass fraction of 99.70% was detected at a temperature of 10 degrees C, a light intensity of 431 mu mol m-2 s-1, and an aeration rate of 3.0 L min-1. The total fatty acid consisted of a major percentage of MUFAs (46.1-56.6%), followed by SFAs (39.5-53.2%), and PUFAs (0-12.5%) pro-viding a CN index of 59.3, IV of 70 g I2/100 g FAMEs, DU of 71%, CFPP of -4.5 degrees C, and SV and HHV of 205 mg KOH g-1 and 39.5 MJ kg-1, respectively, meeting the requirements of EN 14214 and ASTM D6751. Additionally, the maximum total phenol content value of 3.53 mg Gallic acid equivalent g-1 dry weight was obtained via a Folin-Ciocalteu assay at 22 degrees C, 98 mu mol m-2s-1, and 3.0 L min-1, while, the maximum total antioxidant capacity of 16.27 mu mol Trolox equivalent an-tioxidant capacity g-1 dry weight was observed with the help of the ABTS method over a broader cultivation condition range. Hence, P. tricornutum can be considered as a potential feedstock for biofuel and value-added bioproduct production offering a novel integrated lab-scale biorafina-tion process.<br/>Acknowledgements The current study was carried out with the financial support of TUBITAK 1002 as Project (No: 213M623) and BIDEB 2211/A Na-tional PhD Scholarship Program, EBILTEM as Project (No: 2015/BIL/016) , and Ege University Office of Scientific Research Projects (No: 12-MUH-031, 13-MUH-032, 15-MUH-034, and 17-MUH-043) .<br/>