Eliminating the original cargos of glioblastoma cell-derived small extracellular vesicles for efficient drug delivery to glioblastoma with improved biosafety

• DOI: 10.1016/j.bioactmat.2022.02.013
• Publication Date: 2022-03-16T21:29:36Z
• Authors: Yuhang Guo; Guowen Hu; Yuguo Xia; HaiYan Li; Ji Yuan; Juntao Zhang; Yu Chen; Hua Guo; Yunlong Yang; Yang Wang; Zhifeng Deng
• Keywords: Nanocarriers; Biosafety; Extracellular Vesicles

Tumor derived small extracellular vesicles (TsEVs) display a great potential as efficient nanocarriers for chemotherapy because of their intrinsic targeting ability. However, the inherited risks original cargos (like loaded proteins or RNAs) from parent cancer cells in tumor progression severely hinder practical application. In this study, saponin-mediated cargo elimination strategy was established and practiced glioblastoma (GBM) cell-derived (GBM-sEVs). A high eliminating efficacy molecules confirmed by systematic analysis RNAs GBM-sEVs. addition, functions GBM-sEVs to promote GBM vanished after saponin treatment. Moreover, results cellular uptake vivo imaging demonstrated that treatment preserved homotypic ability Thus, we developed an nanocarrier with improved biosafety suppression. Furthermore, doxorubicin (DOX) transported saponin-treated (sa-GBM-sEVs) displayed effective suppression both subcutaneous orthotopic models mouse. Collectively, study provides feasible way avoid protumoral TsEVs can advance clinical application chemotherapy.