ROS-sensitive PD-L1 siRNA cationic selenide nanogels for self-inhibition of autophagy and prevention of immune escape

• DOI: 10.1016/j.bioactmat.2024.08.013
• Publication Date: 2024-08-27T05:32:17Z
• Authors: Jie Gao; Yonghua Zhai; Weihong Lu; Xianghe Jiang; Jingsheng Zhou; Lili Wu; Longhai Du; Chunqing Ou; Xinyi Zhang; Hanliang He; Jian Zhu; Zhengbiao Zhang; Meiyun Li; Yan Wu; Xiangqiang Pan
• Keywords: Cationic polymerization; Imine

In the field of cancer therapy, inhibiting autophagy has emerged as a promising strategy. However, pharmacological disruption can lead to upregulation programmed death-ligand 1 (PD-L1), enabling tumor immune evasion. To address this issue, we developed innovative ROS-responsive cationic poly(ethylene imine) (PEI) nanogels using selenol chemistry-mediated multicomponent reaction (MCR) technology. This procedure involved simple mixing low-molecular-weight PEI (LMW PEI), γ-selenobutylacetone (γ-SBL), and glycol) methacrylate (PEGMA). Through high-throughput screening, constructed library A