3D bioprinted functional skeletal muscle models have potential applications for studies of muscle wasting in cancer cachexia

• DOI: 10.1016/j.bioadv.2023.213426
• Publication Date: 2023-04-19T15:50:30Z
• Authors: Andrea García-Liz...; Aranzazu Villasante; Jose Antonio Lope...; Marta Flandez; M. Carmen Soler-V...; Dolors Serra; Laura Herrero; Ana Sagrera; Alejo Efeyan; Josep Samitier
• Keywords: Biomaterial

Acquired muscle diseases such as cancer cachexia are responsible for the poor prognosis of many patients suffering from cancer. In vitro models needed to study underlying mechanisms those pathologies. Extrusion bioprinting is an emerging tool emulate aligned architecture fibers while implementing additive manufacturing techniques in tissue engineering. However, designing bioinks that reconcile rheological needs and biological requirements a challenging matter. Here we formulate biomaterial with dual crosslinking modulate physical properties bioprinted models. We design 3D resemble mechanical native show improved proliferation high maturation differentiated myotubes suggesting GelMA-AlgMA-Fibrin possesses myogenic properties. The electrical stimulation model confirmed contractile capability enhanced formation sarcomeres. Regarding functionality models, they served platforms recapitulate skeletal wasting produced by cachexia. genetic expression demonstrated better resemblance muscular biopsies cachectic mouse Altogether, this aimed fabricate manipulable non-costly, fast feasible manner.