The selective modulation of endothelial cell mobility on RGD peptide containing surfaces by YIGSR peptides

• DOI: 10.1016/j.biomaterials.2004.02.012
• Publication Date: 2004-03-12T10:20:08Z
• Authors: M.H Fittkau; P Zilla; D Bezuidenhout; M.P Lutolf; P Human; J.A Hubbell; N Davies
• Keywords: 0301 basic medicine; Extracellular Matrix Proteins; Dose-Response Relationship, Drug; Tissue Engineering; Endothelial Cells; Blood Vessel Prosthesis; Polyethylene Glycols; 03 medical and health sciences; Coated Materials, Biocompatible; Biomimetic Materials; Cell Movement; Antineoplastic Combined Chemotherapy Protocols; Materials Testing; Cell Adhesion; Humans; Adsorption; Cell Adhesion Molecules; Oligopeptides; Cells, Cultured; Protein Binding

The ability of the biomimetic peptides YIGSR, PHSRN and RGD to selectively affect adhesion and migration of human microvascular endothelial cells (MVEC) and vascular smooth muscle cells (HVSMC) was evaluated. Cell mobility was quantified by time-lapse video microscopy of single cells migrating on peptide modified surfaces. Polyethylene glycol (PEG) hydrogels modified with YIGSR or PHSRN allowed only limited adhesion and no spreading of MVEC and HVSMC. However, when these peptides were individually combined with the strong cell binding peptide RGD in PEG hydrogels, the YIGSR peptide was found to selectively enhance the migration of MVEC by 25% over that of MVEC on RGD alone (p<0.05). No corresponding effect was observed for HVSMC. This suggests that the desired response of specific cell types to tissue engineering scaffolds could be optimized through a combinatory approach to the use of biomimetic peptides.