Ex vivo expansion and functional activity preservation of adult hematopoietic stem cells by a diarylheptanoid from Curcuma comosa

• DOI: 10.1016/j.biopha.2021.112102
• Publication Date: 2021-08-30T17:29:11Z
• Authors: Nopmullee Tanhuad; Umnuaychoke Thong...; Nareerat Sutjarit; Ploychompoo Yoosabai; Wittaya Panvongsa; Sirapope Wongniam; Apichart Suksamrarn; Pawinee Piyachatu...; Usanarat Anurathapan; Suparerk Borwornp...; Arthit Chairoungdua; Suradej Hongeng; Kanit Bhukhai
• Keywords: CD90; Ex vivo

Hematopoietic stem cells (HSCs, CD34+ cells) have shown therapeutic efficacy for transplantation in various hematological disorders. However, a large quantity of HSCs is required transplantation. Therefore, strategies to increase HSC numbers and preserve functions through ex vivo culture are critically required. Here, we report that expansion medium supplemented with ASPP 049, diarylheptanoid isolated from Curcuma comosa, cocktail cytokines markedly increased adult cells. Interestingly, phenotypically defined primitive (CD34+CD38-CD90+) were significantly under 049 treatment relative control. also improved two functional properties HSCs, as evidenced by an number CD34+CD38- secondary (self-renewal) the growth colony-forming units assessed colony formation assay (multilineage differentiation). Transplantation cultured into immunodeficient mice demonstrated long-term reconstitution differentiation ability 049-expanded RNA sequencing KEGG analysis revealed Hippo signaling was most likely pathway involved effects 049. These results suggest preservation expanded HSCs. Our findings provide rationale use grow prior disease treatment.