Immunomodulatory assessment of Portulaca oleracea L. extract in a mouse model of colitis

• DOI: 10.1016/j.biopha.2021.112148
• Publication Date: 2021-09-21T05:33:07Z
• Authors: Manal A. Alfwuaires; Abdulmohsen I. Al...; Eman Afkar; Sherine Abdel Salam; Heba Ibrahim Abd ...; Gehan M. Badr
• Keywords: Portulaca

Ulcerative colitis (UC) is a gastrointestinal inflammatory disease with multifactorial pathophysiology. This study aims to investigate the immunomodulatory effect of Portulaca oleracea leaf ethanolic extract (POE) on acetic acid (AA)-induced UC in mice. Experimental animals received oral doses POE (200 mg/kg for 7 days) after an induction by intrarectal AA administration. In mice AA-induced treated POE, results revealed significant modulation body weight and colon length. Moreover, treatment downregulated interleukin 1, 6, 17, tumor necrosis factor-alpha, gamma interferon, nuclear factor-kappa B levels compared group. Furthermore, markedly inhibited histological damage, decreased myeloperoxidase activity reduced fecal calprotectin level These data are consistent reduction total bacterial content colon. Taken together, may reduce colonic inflammation alleviating immune response inhibiting severity colitis. The HPLC analysis resulted identification seven medicinal compounds comprising two phenolic acids (ferulic caffeic acids) five flavonoids (kaempferol, quercetin, rutin, narenginin hesperidin). Subsequent GC-MS ten phytocomponents; major percentages were hexadecenoic acid, methyl ester (29.8119%), α-linolenic (25.8431%), 16-octadecenoic (15.1578%) α-tocopherol (10.7848%). Delta-lactams alkanes minor components. Such natural plant-derived substances their probable synergistic action appear contribute promising therapeutic protocol