Effect of memantine on the survival of an ischemic random skin flap and the underlying mechanism

• DOI: 10.1016/j.biopha.2021.112163
• Publication Date: 2021-09-10T16:15:00Z
• Authors: Weijian Fan; Zhenhua Liu; Jianpeng Chen; Shaodong Liu; Tingxiang Chen; Zhijie Li; Dingsheng Lin
• Keywords: Memantine; Malondialdehyde

Skin flap transplantation is a common wound repair method in orthopedic surgery, but skin necrosis remains problematic. Memantine, an excitatory amino acid receptor antagonist, currently used the treatment of moderate to severe Alzheimer's disease, due its ability promote angiogenesis and reduce oxidative stress. This study investigated effect memantine on survival random flaps Sprague-Dawley (SD) rats. Thirty six male SD rats were divided into control, high-dose (20 mg/kg per day), low-dose (10 day) groups underwent McFarland procedure. Seven days later, was evaluated, The microvascular density neutrophil measured by hematoxylin eosin staining. Lead angiography detect angiogenesis, laser Doppler blood perfusion. Expression levels vascular endothelial growth factor (VEGF), interleukin (IL)-1β, IL-6, tumor (TNF)-α, Toll-like (TLR) 4, nuclear kappa B(NF-κB) Mitogen-activated protein kinase(MAPK)were detected immunohistochemistry. Oxidative stress evaluated measuring malondialdehyde (MDA) superoxide dismutase (SOD). Compared with control group, area especially larger, VEGF expression, density, perfusion, higher Memantine-H group than Memantine-L (P < 0.01). In addition, IL-1β, TNF-α, TLR4, NF-κB, MAPK decreased significantly Memantine can improving supply, promoting inhibiting inflammatory response, reducing ischemia-reperfusion injury.