Targeting NLRP3 inflammasome modulates gut microbiota, attenuates corticospinal tract injury and ameliorates neurobehavioral deficits after intracerebral hemorrhage in mice

• DOI: 10.1016/j.biopha.2022.112797
• Publication Date: 2022-03-10T18:00:09Z
• Authors: Linglong Xiao; Huaping Zheng; Jing Li; Meiqin Zeng; Dian He; Jianhao Liang; Kaijian Sun; Yunhao Luo; Feng Li; Baohong Ping; Wen Yuan; Hongwei Zhou; Qinghua Wang; Haitao Sun
• Keywords: Dysbiosis; Corticospinal tract

Intracerebral hemorrhage (ICH) has a high mortality and disability rate. Fewer studies focus on white matter injury (WMI) after ICH, especially the corticospinal tract (CST) located in spinal cord, which correlates with motor impairments. Recent have shown that gut microbiota dysbiosis occurs ICH. Furthermore, NLRP3 inflammasome can be activated resulting inflammatory cascade reactions aggravating brain injury. However, no direct causal correlation among inhibition, altered microbiota, CST following ICH been reported. This study aimed to investigate effect of MCC950, selective inhibitor, We observed compared sham group, members Firmicutes, such as Faecalibaculum Dubosiella, were depleted + Vehicle whereas Proteobacteria Campilobacterota enriched, Enterobacter Helicobacter. After treatment Bacteroides, Bifidobacterium Paenibacillus relatively abundant flora mice. Moreover, we cervical enlargement MCC950 alleviated it. decreased mNSS score water content Taken together, present showed modulated effectively attenuated ameliorated neurological deficits provided novel report links alteration profound implications for treatment.