Comparison of five diarrhea-predominant irritable bowel syndrome (IBS-D) rat models in the brain-gut-microbiota axis

• DOI: 10.1016/j.biopha.2022.112811
• Publication Date: 2022-03-15T15:18:42Z
• Authors: Haomeng Wu; Kai Zhan; Kehan Rao; Huan Zheng; Shumin Qin; Xudong Tang; Shaogang Huang
• Keywords: Gut–brain axis

The brain-gut-microbiota (BGM) axis is known to be essential for diarrhea-predominant irritable bowel syndrome (IBS-D). In order evaluate the effects of IBS-D rat models (the central sensitization model, peripheral model and compound model) on BGM axis, five were induced in Wistar rats with 4% acetic acid (AD, dissolved 0.4 ml AD 9.6 ultrapure water) + wrap restrain stress (WRS), AD, colorectal distention (CRD), WRS, neonatal maternal separation (NMS). Abdominal withdrawal reflex (AWR) scale scores moisture content feces (MCF) evaluated day completing modeling. Body weight was measured every 7 days during Brain gut peptides, cytokine levels, activity spinal cord neurons, intestinal mucosal barrier function, microbiota determined after induction IBS-D. We found intervention CRD, NMS a similar course axis. Among models, AWR (60 mmHg, 80 mmHg) all increased. levels 5-hydroxytryptamine, corticotropin-releasing factor, substance P, calcitonin gene-related protein serum rapidly increased, whereas that neuropeptide Y decreased. C-fos showed increased neuronal activity. permeability composition structure changed. conclusion, could cause changes but WRS closer post-inflammatory