Pentoxifylline and berberine mitigate diclofenac-induced acute nephrotoxicity in male rats via modulation of inflammation and oxidative stress

• DOI: 10.1016/j.biopha.2022.113225
• Publication Date: 2022-06-04T03:33:53Z
• Authors: Mohammed Alorabi; Simona Cavalu; Hayder M. Al-kura...; Ali I. Al-Gareeb; Gomaa Mostafa-Hedeab; Walaa A. Negm; Amal Youssef; Aya H. El-Kadem; Hebatallah M. Saad; Gaber El-Saber Ba...
• Keywords: Pentoxifylline; Malondialdehyde; Nephrotoxicity; Diclofenac

Nephrotoxicity (NT) is a renal-specific situation caused by different toxins and drugs like non-steroidal anti-inflammatory (NSAIDs). NSAIDs diclofenac (DCF) lead to glomerular dysfunction. Pentoxifylline (PTX) berberine (BER) have antioxidant properties. Thus, the objective of present study was investigate ameliorative effect PTX, BER their combination against DCF-mediated acute NT. Induction NT done via DCF injection (150 mg/kg I.P, for 6 days) in rats. PTX 200 mg/kg, were administrated days prior concurrently with additional days. Acute evaluated biochemically histopathologically measuring blood urea (BU), serum creatinine (SCr), kidney injury molecule-1(KIM-1), integrin (ITG), vitronectin (VTN), interleukin (IL)-18, Neutrophil gelatinase-associated lipocalin (NGAL), filtration rate (GFR), superoxide dismutase (SOD) glutathione (GSH) malondialdehyde (MDA) scoring histopathological alterations. significantly (P < 0.05) attenuated biochemical changes amelioration BU, SCr, KIM-1, ITG, VTN, IL-18, NGAL, GFR, SOD, GSH, MDA The combined effects produced more significant than either or when used alone DCF-induced In conclusion, BTX found potential renoprotective rats inhibiting inflammatory reactions oxidative stress.