Secoisolariciresinol diglucoside Ameliorates Osteoarthritis via Nuclear factor-erythroid 2-related factor-2/ nuclear factor kappa B Pathway: In vitro and in vivo experiments

• DOI: 10.1016/j.biopha.2023.114964
• Publication Date: 2023-06-01T11:36:46Z
• Authors: Zhiwei Zhang; Song Wang; Xuqiang Liu; Yuxin Yang; Yiqin Zhang; Bo Li; Fengfen Guo; Jianhui Liang; Xin Hong; Runsheng Guo; Bin Zhang
• Keywords: MMP3

Osteoarthritis (OA) is an age-related joint disease in which inflammation and extracellular matrix (ECM) degradation play a crucial role the destruction of articular cartilage. Secoisolariciresinol diglucoside (SDG), main lignan wholegrain flaxseed, has been reported to remarkably suppress oxidative stress, may have potential therapeutic value OA. In this study, effect mechanism SDG against cartilage degeneration were verified destabilization medial meniscus (DMM) collagen-induced (CIA) arthritis models interleukin-1β (IL-1β)-stimulated osteoarthritis chondrocyte models. From our experiments, treatment downregulated expression pro-inflammatory factors induced by IL-1β vitro, including inducible nitric oxide synthase (INOS), cyclooxygenase-2 (COX2), tumor necrosis factor (TNF-α), interleukin 6 (IL-6). Additionally, promoted collagen II (COL2A1) SRY-related high-mobility-group-box gene 9(SOX9), while suppressing disintegrin metalloproteinase with thrombospondin motifs 5(ADAMTS5) metalloproteinases 13(MMP13), leads catabolism. Consistently, vivo, identified chondroprotective effects DMM-induced Mechanistically, exerted its anti-inflammation anti-ECM activating Nrf2/HO-1 pathway inhibiting nuclear kappa B (NF-κB) pathway. conclusion, ameliorates progression OA via Nrf2/NF-κB pathway, indicates that for