Unveiling the volatile compounds and antibacterial mechanisms of action of Cupressus sempervirens L., against Bacillus subtilis and Pseudomonas aeruginosa

• DOI: 10.1016/j.biopha.2023.115609
• Publication Date: 2023-10-04T11:47:30Z
• Authors: Samiah Hamad Al-M...; Naoufal El Hachlafi; Mohamed Jeddi; Emad M. Abdallah; Hamza Assaggaf; Ahmed Qasem; Learn-Han Lee; Jodi Woan-Fei Law; Mohammed Aladhadh; Sulaiman Mohammed...; Abdelhakim Bouyahya; Hanae Naceiri Mrabti
• Keywords: Minimum bactericidal concentration

Cupressus sempervirens is a known traditional plant used to manage various ailments, including cancer, inflammatory and infectious diseases. In this investigation, we aimed explore the chemical profile of essential oil (CSEO) as well their antibacterial mode action. The volatile components were characterized using gas chromatography coupled mass spectrometer (GC-MS). results revealed remarkable properties EO derived from C. sempervirens. GC-MS analysis indicated that by δ-3-carene (47.72%), D-limonene (5.44%), β-pinene (4.36%), β-myrcene (4.02%). exhibited significant inhibitory effects against range bacteria, Staphylococcus aureus ATCC 29213, Bacillus subtilis 13048, cereus (Clinical isolate), Pseudomonas aeruginosa 27853, Escherichia coli 25922. These surpassed those conventional antibiotics. Furthermore, demonstrated low minimum concentrations (MICs) bactericidal (MBCs), indicating its nature (MBC/MIC < 4.0). Time-kill kinetics showed CSEO was particularly effective at 2 × MIC doses, rapidly reduced viable count B. P. within 8 h. This suggests acts quickly efficiently. cell membrane permeability test further impact on relative conductivity aeruginosa, both concentrations. observations suggest disrupts bacterial membrane, thereby influencing growth viability. Additionally, integrity addition cultures resulted in release proteins cells. affects structural anti-biofilm assay confirmed efficacy potent agent. It oil's ability inhibit quorum sensing, crucial mechanism for biofilm formation, competitive performance compared tested