Polydatin protects against calcium oxalate crystal-induced renal injury through the cytoplasmic/mitochondrial reactive oxygen species-NLRP3 inflammasome pathway

• DOI: 10.1016/j.biopha.2023.115621
• Publication Date: 2023-10-02T16:09:32Z
• Authors: Jiannan Liu; Jianlin Huang; Bo Gong; Songtao Cheng; Yadong Liu; Yaodong Chen; Qiang Feng; Jun Li; Mingxing Qiu; Gui Yu; Yong Liao
• Keywords: Mitochondrial ROS; Proinflammatory cytokine; Pyroptosis

Oxidative stress and inflammatory responses are critical factors in calcium oxalate (CaOx) crystal-induced renal injury. Reactive oxygen species (ROS) usually produced the cytoplasm mitochondria trigger priming activation of NLRP3 inflammasome, thereby regulating cytokines inflammation. Polydatin is a plant rhizome extract with anti-inflammatory, antioxidant, antitumor effects. However, it remains not clear whether how these pathophysiological processes exists CaOx injury.Here, we measured expression IL-18, IL-1β, intracellular mitochondrial ROS (mtROS) levels relevant morphological changes treated tubular epithelial cells (TECs) stone-forming rats. The study further explored action mtROS on damage, beneficial effects pathway polydatin.We verified that cytoplasmic upregulation promoted stimulating IL-18/1β maturation activation. can relieve oxidative damage by decreasing ROS. We demonstrated main target for polydatin to exert inflammasome-regulating function. inhibition effectively TECs kidney caused crystal.These findings provide new insight into relationship between inflammation nephrolithiasis show polydatin-mediated anti-inflammatory antioxidative protection therapeutic strategy for, but limited to, crystalline nephropathy.