Carboxylated mesoporous silica nanoparticle-nucleic acid chimera conjugate-assisted delivery of siRNA and doxorubicin effectively treat drug-resistant bladder cancer.

• DOI: 10.1016/j.biopha.2024.117185
• Publication Date: 2024-07-24T21:01:55Z
• Authors: Jintao Yang; Biao Liu; Qi Wang; Hao Yan; Guangping Li; Xu Wang; Zhenhua Shang; Tongwen Ou; Wen Chen
• Keywords: Chimera (genetics); Aptamer

Chemotherapy is the main treatment for bladder cancer, but drug resistance and side effects limit its application therapeutic effect. Herein, we constructed doxorubicin (DOX)/COOH-mesoporous silica nanoparticle/polyethylenimine (PEI)/nucleic acid chimeras (DOX/MSN/Chimeras) to reduce toxicity of chemotherapy drugs cancer cells. Transmission electron microscopy showed that PEI was coated on DOX/MSN/BSA nanoparticles with a diameter about 150 nm. DOX/MSN/PEI could control DOX release over 48 h, sudden rate significantly lower than DOX/MSN. Immunohistochemical results DOX/MSN/Chimera specifically bound cells, markedly inhibited PI3K expression proliferation DOX-resistant promoted apoptosis drug-resistant which superior DOX/MSN/Aptamer or We further carried out animal experiments found volume transplanted tumors in vivo. Compared group, decreased proportion apoptotic cells highly increased. Through histological observation kidneys lungs, believed can effectively damage normal tissues. In conclusion, COOH-MSN/nucleic chimera conjugate targeted delivery siRNA anti-cancer drugs. Our study provides new method personalized cancer.