Enhanced ammonia nitrogen removal from actual rare earth element tailings (REEs) wastewater by microalgae-bacteria symbiosis system (MBS): Ratio optimization of microalgae to bacteria and mechanism analysis

• DOI: 10.1016/j.biortech.2022.128304
• Publication Date: 2022-11-09T17:10:26Z
• Authors: Zhuochao Liu; Dan Cui; Yuanqi Liu; Haiyu Wang; Liming Yang; Houxing Chen; Genping Qiu; Zhensheng Xiong; Penghui Shao; Xubiao Luo
• Keywords: Bacteria; Ammonia; Nitrogen; Microalgae; Denitrification; 0211 other engineering and technologies; Biomass; 02 engineering and technology; Wastewater; Symbiosis; 6. Clean water

Microalgae-bacteria symbiosis system (MBS) appear to be a promising way for treating the rare earth elements (REEs) wastewater due to the natural symbiotic interactions between microalgae and bacteria. Herein, we investigated the effect of different inoculation ratios of microalgae and bacteria including 3:1 (MB_1), 1:1 (MB_2) and 1:3 (MB_3) on NH4+-N removal from REEs wastewater and analyzed the corresponding biological mechanism. The NH4+-N removal rate with MB_3 reached 17.69 ± 0.45 mg NH4+-N/L d-1, which was 2.58 times higher than that in single microalgae system. The results were further verified in continuous feeding photobioreactors and kept stable for 100 days. Metagenomic analysis revealed that the abundance of genes related to microalgae assimilation increased by 14 %-50 % in answer to photosynthesis and NH4+-N absorption, while that related to nitrification apparently dropped, indicating that MBS was a sustainable method capable of enhancing NH4+-N removal from REEs wastewater.