Highly selective optical and reversible dual-path chemosensor for cyanide detection and its application in live cells imaging

• DOI: 10.1016/j.bios.2017.02.006
• Publication Date: 2017-02-06T17:30:45Z
• Authors: Karunesh Keshav; Pritam Torawane; Mukesh Kumar Kumawat; Kundan Tayade; Suban K. Sahoo; Rohit Srivastava; Anil Kuwar
• Keywords: Models, Molecular; Cyanide; Metal-Complex; Anion; Biosensing Techniques; Dft; 01 natural sciences; Cell Line; Mice; 2-Photon Absorption; Cellular Imaging; Limit of Detection; Nitriles; Fluorescent Turn-On Sensor; Animals; Benzothiazoles; Ratiometric Chemosensor; Aqueous-Media; Sensor; Fluorescent Dyes; Ions; Cyanides; Optical Imaging; Water; Fibroblasts; 0104 chemical sciences; Recognition; Spectrometry, Fluorescence; Biocompatibility; Hydrogen

A new biocompatible fluorescent receptor 1 was synthesized by conjugating diaminomaleonitrile (DMN) with benzothiazole unit, and characterized by single X-ray. In DMF/water (1:1, v/v), the receptor 1 showed a selectivity turn-on fluorescence at 517nm in the presence of CN-. Receptor 1 showed a detection limit down to 0.16µM without any interference from other tested anions. The reversibility and reusability of 1 for the detection of CN- ion was also tested for five cycles indicating the probe 1 could be used in a reversible manner. Importantly, the receptor 1 showed excellent cells viability and was successfully applied for the detection of CN- in live mouse fibroblast cells L929 cells.