Lab-made flexible third-generation fructose biosensors based on 0D-nanostructured transducers

• DOI: 10.1016/j.bios.2023.115450
• Publication Date: 2023-06-15T20:21:52Z
• Authors: Filippo Silveri; Davide Paolini; Flavio Della Pelle; Paolo Bollella; Annalisa Scroccar...; Yohei Suzuki; Eole Fukawa; Keisei Sowa; Cinzia Di Franco; Luisa Torsi; Dario Compagnone
• Keywords: Direct electron transfer; Enzyme-based sensors; Flexible amperometric biosensors; Fructose dehydrogenase; Nanomaterials; Point-of-care/needs devices; Reproducibility of Results; Water; Enzyme-based sensors; Fructose; Biosensing Techniques; Flexible amperometric biosensors; 540; 7. Clean energy; Direct electron transfer, Point-of-care/needs devices, Nanomaterials, Flexible amperometric biosensors, Fructose dehydrogenase, Enzyme-based sensors; Fructose dehydrogenase; Direct electron transfer; Point-of-care/needs devices; Oxidoreductases; Electrodes; Nanomaterials

Herein, we report a scalable benchtop electrode fabrication method to produce highly sensitive and flexible third-generation fructose dehydrogenase amperometric biosensors based on water-dispersed 0D-nanomaterials. The electrochemical platform was fabricated via Stencil-Printing (StPE) insulated xurography. Carbon black (CB) mesoporous carbon (MS) were employed as 0D-nanomaterials promoting an efficient direct electron transfer (DET) between (FDH) the transducer. Both nanomaterials prepared in water-phase sonochemical approach. nano-StPE exhibited enhanced electrocatalytic currents compared conventional commercial electrodes. enzymatic sensors exploited for determination of D-fructose model solutions various food biological samples. StPE-CB StPE-MS integrated showed appreciable sensitivity (∼150 μA cm-2 mM-1) with μmolar limit detection (0.35 0.16 μM, respectively) extended linear range (2-500 1-250 respectively); selectivity biosensors, ensured by low working overpotential (+0.15 V), has been also demonstrated. Good accuracy (recoveries 95 116%) reproducibility (RSD ≤8.6%) achieved urine proposed approach because manufacturing versatility electro-catalytic features water-nanostructured 0D-NMs opens new paths affordable customizable FDH-based bioelectronics.