Colorimetric biosensor for the naked-eye detection of ovarian cancer biomarker PDGF using citrate modified gold nanoparticles

• DOI: 10.1016/j.biosx.2022.100142
• Publication Date: 2022-04-26T01:33:50Z
• Authors: Mohd Rahil Hasan; Pradakshina Sharma; Roberto Pilloton; Manika Khanuja; Jagriti Narang
• Keywords: Aptamer; Absorbance

Ovarian cancer is one of the fatal diseases in women. However, it can be cured if early-stage diagnosis and suitable treatment given on time. The goal research to produce nano-biosensing technology for early identification ovarian cancer. In this regard, a rapid colorimetric nano-biosensor was constructed employing gold nanoparticles target platelet-derived growth factor (PDGF), circulating biomarker that up-regulated plasma prevalent findings were supported by spectroscopy analysis characterized using SEM (Scanning electron microscopy & XRD (X-ray diffraction). proposed principle, (AuNPs) are mixed with PDGF specific aptamer employed identify screening changes color as well absorbance Aptamer AuNPs caused aggregation. from pinkish light purple at higher level concentration. Signal-output exhibited linear range 0.01–10 μg/ml under optimum conditions. change seen when concentration low 0.01 μg/ml. Also, developed technique successfully applied artificial serum which have many components biofluids. results we presented imply practical application diagnosis, exhibiting its benefits reliability, selectivity, reproducibility.