A portable, low-cost and high-throughput electrochemical impedance spectroscopy device for point-of-care biomarker detection

• DOI: 10.1016/j.biosx.2022.100301
• Publication Date: 2022-12-20T17:30:15Z
• Authors: Xuanjie Ye; Tianxiang Jiang; Yuhao Ma; Daniel To; Shuren Wang; Jie Chen
• Keywords: Crest factor; Linearity; SIGNAL (programming language)

This paper presents a portable, fast and accurate electrochemical impedance spectroscopy (EIS) device with 8-well interdigitated electrode chips for biomarker detection. The design adopts low crest factor multisine signal synthesis at frequencies (<1 kHz) single-tone signals high (>1 kHz), which significantly increases measurement speed without sacrificing accuracy. In addition, the excitation amplitude of 10 mV preserves linearity protects biosamples. system achieved an average magnitude accuracy error 0.30% in frequency range interest it requires only 0.46 s to scan 28 points from Hz 1 MHz. Experiments were conducted test capability detect antibodies against SARS-CoV-2. Gold nanoparticles bound protein G (GNP-G) employed as conjugated secondary antibody probe anti-SARS-CoV-2 IgG serum. A highly statistical significance (p = 7×10−6) could be found data kHz. alteration caused by GNP-G positive negative groups 27.2%±13.6% 4.1%±1.7%, respectively. results imply that proposed enables rapid COVID-19 Moreover, EIS GNPs have potential modified other biomarkers.