Portable optical biosensor for point-of-care monitoring of salivary glucose using a paper-based microfluidic strip

• DOI: 10.1016/j.biosx.2024.100452
• Publication Date: 2024-02-10T03:25:52Z
• Authors: Shweta Panwar; Paulami Sarkar; D. Syed Kasim; Raksha Anand; Akanksha Priya; Shyam Prakash; Sandeep Kumar Jha
• Keywords: Chromogenic; Glucose oxidase; Point of care; ABTS

The manuscript describes a technique for fabrication and validation of standalone handheld optical biosensor designed non-invasive monitoring glucose through saliva. In this cost-effective process, 3D-printed test strip was filled with sieving paste comprising cellulose, polyethylene glycol (PEG), polyvinyl alcohol (PVA) glycerol, onto which, oxidase-peroxidase (GOD-POD) enzymes 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) chromogenic dye were co-immobilized. enzymatic reaction produced H2O2 as by-product which the ABTS reacted, leading to colour change on detection zone detected by developed glucometer. in-house meter included an optically isolated section in its structure inserting prevent interference from ambient light conditions. exhibited broad range 28–204 mg/dL concentration, sensitivity 26.89 count/mg/dL limit (LOD) at 28 mg/dL, within response time 120 seconds. device along strips validated clinical samples, comparing salivary levels (SGL) blood (BGL) using commercial glucometer i.e., Accu-Chek Active. Student's t-test data yielded p-values 0.018, 0.01, 0.008, 0.003 fasting post-prandial samples non-diabetic diabetic patients respectively, represents significant correlation. also passed Clarke's error grid analysis is hence considered medically acceptable. low-cost simple-to-use saliva-based should be ideally suited mass screening diabetes well day-to-day health check-ups painless manner.