Metal-organic frameworks as an active substrate for cell-interaction studies and cell-on-a-chip platforms

• DOI: 10.1016/j.biosx.2024.100487
• Publication Date: 2024-05-11T16:09:06Z
• Authors: Huijie Jiang; Ziyu Gao; Claudia Lubrano; Claudia Latte Bovio; Henning Bommes; Andrea Kauth; Lea Baumann; Bo Cheng; Divagar Murugan; Joachim Knoch; Rainer Waser; Sven Ingebrandt; Francesca Santoro; Vivek Pachauri
• Keywords: Biocompatibility

Metal-organic frameworks (MOFs) are an emerging class of materials with immense biomedical potential for their unique interactions biological and organic materials. In this work, we select two candidate two-dimensional (2D) MOF systems based on Fe3+ Ni2+ metal centers 2-aminoterephthalate acid ligand (Fe-MOF Ni-MOF) evaluate performance as active interface study cell-interactions. 2D Fe-MOF Ni-MOF were synthesized onto hydroxyl-modified gold glass substrates using a layer-by-layer liquid-phase-epitaxy (LbL-LPE) growth at room temperature used (Fe-MOF/glass, Fe-MOF/Au, Ni-MOF/glass Ni-MOF/Au, respectively) MTT cell-proliferation reactive oxygen species tests the PC-12 cell-line in order to investigate biocompatibility. Immunostaining morphological analyses cells interfaces suggested stronger cell-substrate interaction comparison further characterized Electrical Cell-substrate Impedance Sensing (ECIS) technique, here first time, employed cell attachment, spreading proliferation Fe-MOF. The showed superior long-term stability culture medium by recording impedance over 24 hours, crucial monitor cell-dynamics solid-liquid interface. A significant increase interfacial was observed ECIS, due adhering Fe-MOF, which also confirmed focused ion beam etching followed scanning electron microscopy. Our novel findings, therefore, suggest MOFs highly suitable platform cell-related electrical techniques potentially pave way future use bioelectronics biosensor applications.