The spontaneous immortalization probability of mammalian cell culture strains, as their proliferative capacity, correlates with species body mass, not longevity

• DOI: 10.1016/j.bj.2023.100596
• Publication Date: 2023-05-05T02:38:30Z
• Authors: Matteo Perillo; Angela Punzo; Cristiana Caliceti; Christian Sell; Antonello Lorenzini
• Keywords: Mammals; Medicine (General); QH301-705.5; Partial correlation; Longevity; Cell Culture Techniques; 3. Good health; Body mass; Cell culture immortalization; Longevity; Partial correlation; Phylogenetic regression; Replicative senescence; R5-920; Body mass; Neoplasms; Animals; Replicative senescence; Original Article; Biology (General); Phylogenetic regression; Cell culture immortalization; Phylogeny; Probability

The Peto's paradox consists in the observation that individuals from long-lived and large animal species do not experience a higher cancer incidence, despite being exposed for longer time to possibility of accumulating mutations having more target cells phenomenon. existence this has been recently confirmed (Vincze et al., 2022). Concurrently, robust evidence published longevity involves convergent evolution cellular mechanisms prevent accumulation (Cagan It remains unclear which are critical allow body mass while keeping at bay.Adding existing data linking replicative potential (Lorenzini 2005), we have grown total 84 skin fibroblast cell strains 40 donors 17 mammalian analyzed their Hayflick's limit, i.e., senescent plateau, eventual spontaneous immortalization escape. correlation capacity with longevity, metabolism assessed through phylogenetic multiple linear regression (MLR).The probability is negatively related mass. new evaluation additional about strengthen our previous observation, confirming stable extended proliferation strongly correlated rather than lifespan.The relation between suggests need evolve stringent control genetic stability during