Comprehensive insight into the alterations in the gut microbiome and the intestinal barrier as a consequence of iron deficiency anaemia

• DOI: 10.1016/j.bj.2024.100701
• Publication Date: 2024-01-26T21:02:24Z
• Authors: Ana Soriano-Lerma; María García-Burgos; Wiley Barton; María José M. Alf...; Jorge Valentín Cr...; Miguel Soriano; Inmaculada López-...; Paul D. Cotter; José A. García-Sa...
• Keywords: Intestinal epithelium

Iron deficiency is the top leading cause of anaemia, whose treatment has been shown to deteriorate gut health. However, a comprehensive analysis intestinal barrier and microbiome during IDA have not performed date. This study aims delve further into these two aspects, which will mean step forward minimising negative impact iron supplements on was experimentally induced in an animal model. Shotgun sequencing used analyse colonic region, while studied through histological analyses, mRNA (RNA-Seq), qPCR immunofluorescence. Determinations lipopolysaccharide (LPS) bacteria-specific immunoglobulins were assess microbial translocation. Microbial metabolism colon shifted towards increased production certain amino acids, short chain fatty acids nucleotides, with Clostridium species being enriched IDA. Structural alterations epithelium by analysis. RNA-Seq revealed downregulation extracellular matrix-associated genes proteins overall underdeveloped epithelium. Increased levels serum LPS immune response against dysbiotic bacteria support impairment integrity negatively impacts barrier, triggering emphasizes deterioration health fact that it should be addressed when treating disease.