A convenient one-step synthesis of l-aminotryptophans and improved synthesis of 5-fluorotryptophan

• DOI: 10.1016/j.bmcl.2008.07.053
• Publication Date: 2008-07-18T09:28:54Z
• Authors: Michael Winn; Abhijeet Deb Roy; Sabine Grüschow; Raj S. Parameswaran; Rebecca J.M. Goss
• Keywords: Drug Compounding; Chemistry, Organic; Tryptophan; 540; Biochemistry; 01 natural sciences; 0104 chemical sciences; Freeze Drying; Drug Stability; Models, Chemical; Drug Design; Tryptophan Synthase; Biotransformation

A one-pot biotransformation for the generation of a series of L-aminotryptophans using a readily prepared protein extract containing tryptophan synthase is reported. The extract exhibits remarkable stability upon freeze-drying, and may be stored and used for long periods after its preparation without significant loss of activity.