Measuring flow-mediated protein drift across stationary supported lipid bilayers

• DOI: 10.1016/j.bpj.2023.03.042
• Publication Date: 2023-04-05T15:28:05Z
• Authors: Amanda M. Ratajczak; Sreeja Sasidharan; Xaymara I. Rivera...; Ethan J. Miller; Larissa Socrier; Autumn A. Anthony; Aurelia R. Honerk...
• Keywords: Membranes; Lipid Bilayers; Cell Membrane; Reproducibility of Results; Membrane Proteins; Article

Fluid flow near biological membranes influences cell functions such as development, motility, and environmental sensing. Flow can laterally transport extracellular membrane proteins located at the cell-fluid interface. To determine whether this contributes to signaling in cells, quantitative knowledge of forces acting on is required. Here, we demonstrate a method for measuring flow-mediated lateral lipid-anchored proteins. We rupture giant unilamellar vesicles form discrete patches supported inside rectangular microchannels then allow bind upper surface membrane. While applying flow, observe formation protein concentration gradients that span patch. By observing how these dynamically respond changes applied shear stress, mobility protein. use simplified model our method's sensitivity reproducibility. Our intention was design quantitative, reliable analysis will compare variety proteins, lipid anchors, systems living cells.