Fabrication of silver nanoparticles by a diethylene triamine-hyaluronic acid derivative and use as antibacterial coating

• DOI: 10.1016/j.carbpol.2022.119861
• Publication Date: 2022-07-13T06:02:58Z
• Authors: Annalisa Martorana; Giovanna Pitarresi; Fabio Salvatore P...; Valentina Catania; Domenico Schillaci; Nicolò Mauro; Calogero Fiorica; Gaetano Giammona
• Keywords: Diethylenetriamine; Silver; Ag nanoparticles; Hyaluronic acid; DEET; Metal Nanoparticles; 02 engineering and technology; Anti-Bacterial Agents; 3. Good health; Ag nanoparticles, Antibacterial activity, Diethylenetriamine, Hyaluronic acid, Medical device infections; Settore CHIM/09 - Farmaceutico Tecnologico Applicativo; Polyamines; Antibacterial activity; Medical device infections; Hyaluronic Acid; 0210 nano-technology

In this work a synthetic protocol for the functionalization of hyaluronic acid with diethylenetriamine (DETA) was standardized. HA-DETA derivatives were characterized by NMR and proton carbon correlation analysis HSQC and HMBC to confirm chemical structure. A selected derivative was used to set up a green fabrication procedure for HA-DETA capped silver nanoparticles with the aim to achieve a polymeric based coating with potential application in the treatment of medical devices associated infections. Data from UV-visible spectroscopy, electron scanning and transmission microscope (STEM), photoelectric spectroscopy (XPS) and rheological characterization were combined to characterize the HA-DETA/Ag nanocomposites. HA-DETA stabilized Ag nanoparticles (10-30 nm) were obtained through an UV accelerated production. The viability of MC3T3-E1 was analyzed with the aim of designing a cytocompatible antimicrobial coating. Antibacterial and antibiofilm activity of HA-DETA/Ag nanocomposites have been tested in vitro against Staphylococcus aureus and Pseudomonas aeruginosa both in culture plates than on titanium specimens.