Altered expression of early cardiac marker genes in circulating cells of patients with hypertrophic cardiomyopathy

• DOI: 10.1016/j.carpath.2007.04.004
• Publication Date: 2007-06-21T11:08:39Z
• Authors: Joanna E. Kontaraki; Fragiskos I. Part...; Alexandros P. Pat...; Ioannis K. Karalis; Panos E. Vardas
• Keywords: Adult; Homeodomain Proteins; Male; Myosin Heavy Chains; MEF2 Transcription Factors; Heart Ventricles; MADS Domain Proteins; Cardiomyopathy, Hypertrophic; Middle Aged; GATA4 Transcription Factor; 3. Good health; 03 medical and health sciences; 0302 clinical medicine; Gene Expression Regulation; Myogenic Regulatory Factors; Case-Control Studies; Homeobox Protein Nkx-2.5; Leukocytes, Mononuclear; Humans; Female; Hypertrophy, Left Ventricular; Biomarkers; Aged

Early cardiac marker genes, such as cardiac-specific homeobox (Csx/Nkx2.5), myocardin, homeodomain only protein, GATA4, and myocyte enhancer factor 2C, are thought to participate in cardiomyocyte differentiation and to contribute to heart hypertrophy in animal models. In this study, we investigated whether the expression of early cardiac genes is altered in the peripheral blood of patients with hypertrophic cardiomyopathy.Peripheral blood mononuclear cells were isolated from 30 consecutive hypertrophic cardiomyopathy patients and 20 healthy controls, and gene expression was determined by quantitative real-time reverse transcription-polymerase chain reaction.Csx/Nkx2.5, myocardin, and GATA4 expressions were significantly higher in hypertrophic cardiomyopathy patients by 5.14+/-0.89 (P<.001), 1.65+/-0.21 (P<.05), and 2.04+/-0.41 (P<.04) times, respectively, while homeodomain only protein showed a fourfold decrease in expression (P<.02) compared to controls. In addition, expression of the differentiation-specific marker genes beta-myosin heavy chain and smooth muscle myosin heavy chain was significantly higher in hypertrophic cardiomyopathy patients by 3.72+/-0.82 (P<.02) and 2.57+/-0.72 (P<.05) times, respectively, compared to controls. Myocyte enhancer factor 2C expression was not different between patients and controls. Furthermore, increased expression of GATA4, myocardin, and beta-myosin heavy chain positively correlated with increased left ventricular mass.In conclusion, we found altered expressions of early cardiac marker genes and differentiation-specific marker genes in peripheral blood mononuclear cells of hypertrophic cardiomyopathy patients compared to control individuals, possibly reflecting changes in response to disease.