Pectin encapsulated novel nanocomposite augments wound healing in Sprague Dawley rats

• DOI: 10.1016/j.carpta.2023.100370
• Publication Date: 2023-09-26T16:35:46Z
• Authors: Kunal Kishore; Saranga Rajesh; Sneha Sivadas; N. Selvasudha; R. Barathidasan; Hannah R. Vasanthi
• Keywords: HaCaT

The complexity of the tissue remodeling process makes treatment options for wound healing inadequate. Herein, potential wound-healing activity a novel nanocomposite with pectin and keratin incorporating silver nanoparticles ferulic acid has been evaluated. Silver concentration, secondary structural stability keratin, presence nano as complex molecule within were confirmed by XPS, CD spectroscopy, ESI-MS analysis, respectively. effects on cell migration role apoptosis in studied scratch assay (A.O./EtBr) staining, Subsequently, vivo studies carried out male Sprague Dawley rats. Wound tracings measured exhibited significant contraction. Further, relative gene expression wound–healing biomarkers MMP2, MMP8, KGF increased considerably 75mg test group compared to negative control indicating faster epithelialization phase healing. Histopathological observations revealed remarkable skin regeneration group. Altogether, at dose exhibits enhanced capability, reepithelialization, than standard, possibly due synergistic influence natural biomolecules. Therefore it is concluded that NC accelerated no toxicity.