Self-assembled chitosan-sodium usnate drug delivery nanosystems: Synthesis, characterization, stability studies, in vitro cytotoxicity and in vivo biocompatibility against 143 B cells

• DOI: 10.1016/j.carpta.2023.100373
• Publication Date: 2023-09-29T06:19:43Z
• Authors: Benedetta Brugnoli; Alessia Mariano; Beatrice Simonis; Cecilia Bombelli; Simona Sennato; Antonella Piozzi; Vincenzo Taresco; Veeren M. Chauhan; Steven M. Howdle; Anna Scotto d'Abusco; Iolanda Francolini
• Keywords: Biocompatibility

Polymers are among the most studied materials as drug carriers, due to their tunable chemical structure and ability self-assemble give different types of nanostructures. In this study, chitosan (CS) nanoparticles (NPs) were investigated carriers for anticancer sodium usnate (NaU) treatment osteosarcoma (OS) is prevalent primary malignant bone sarcoma in pediatric adolescent patients. CS nano-assembling was induced by electrostatic interactions with anionic cross-linker tripolyphosphate, thus obtaining stable nanosystems a high encapsulation efficiency. Importantly, reduction NaU hepatotoxicity when encapsulated NPs compared free evidenced, suggesting that may have protective role against liver damage. Unfortunately, also reduced toxicity versus 143B cells NaU. Nevertheless, CS:NaU5x (0.312 mg/mL) found decrease viability after 48 h 72 without being hepatotoxic. Interestingly, system stimulated Maspin production, an agent known its tumour suppressor properties. Relevant synergistic activity between promoting stimulation found. That suggests potential systems reduce invasiveness type cancer.