Single-cell spatial transcriptome reveals cell-type organization in the macaque cortex

• DOI: 10.1016/j.cell.2023.06.009
• Publication Date: 2023-07-12T14:28:53Z
• Authors: Ao Chen; Yidi Sun; Ying Lei; Chao Li; Sha Liao; Juan Meng; Yiqin Bai; Zhen Liu; Zhifeng Liang; Zhiyong Zhu; Nini Yuan; Hao Yang; Zihan Wu; Feng Lin; Kexin Wang; Mei Li; Shuzhen Zhang; Meisong Yang; Tianyi Fei; Zhenkun Zhuang; Yiming Huang; Yong Zhang; Yuanfang Xu; Luman Cui; Ruiyi Zhang; Lei Han; Xing Sun; Bichao Chen; Wenjiao Li; Baoqian Huangfu; Kailong Ma; Jianyun Ma; Zhao Li; Yikun Lin; He Wang; Yanqing Zhong; Huifang Zhang; Qian Yu; Yaqian Wang; Xing Liu; Jian Peng; Chuanyu Liu; Wei Chen; Wentao Pan; Yingjie An; Shihui Xia; Yanbing Lu; Mingli Wang; Xinxiang Song; Shuai Liu; Zhifeng Wang; Chun Gong; Xin Huang; Yue Yuan; Yun Zhao; Qinwen Chai; Xing Tan; Jianfeng Liu; Mingyuan Zheng; Shengkang Li; Yaling Huang; Yan Hong; Zirui Huang; Min Li; Mengmeng Jin; Yan Li; Hui Zhang; Suhong Sun; Li Gao; Yinqi Bai; Mengnan Cheng; Guohai Hu; Shiping Liu; Bo Wang; Bin Xiang; Shuting Li; Huanhuan Li; Mengni Chen; Shiwen Wang; Minglong Li; Weibin Liu; Xin Liu; Qian Zhao; Michael Lisby; Jing Wang; Jiao Fang; Yun Lin; Qing Xie; Zhen Liu; Jie He; Huatai Xu; Wei Huang; Jan Mulder; Huanming Yang; Yangang Sun; Mathias Uhlen; Muming Poo; Jian Wang; Jianhua Yao; Wu Wei; Yuxiang Li; Zhiming Shen; Longqi Liu; Zhiyong Liu; Xun Xu; Chengyu Li
• Keywords: Cell type

Elucidating the cellular organization of cerebral cortex is critical for understanding brain structure and function. Using large-scale single-nucleus RNA sequencing spatial transcriptomic analysis 143 macaque cortical regions, we obtained a comprehensive atlas 264 transcriptome-defined cell types mapped their distribution across entire cortex. We characterized layer region preferences glutamatergic, GABAergic, non-neuronal types, as well regional differences in cell-type composition neighborhood complexity. Notably, discovered relationship between various region's hierarchical level visual somatosensory systems. Cross-species comparison data from human, macaque, mouse cortices further revealed primate-specific that are enriched 4, with marker genes expressed region-dependent manner. Our provide molecular basis evolution, development, aging, pathogenesis primate brain.