Distinct roles of ICOS and CD40L in human T-B cell adhesion and antibody production

• DOI: 10.1016/j.cellimm.2021.104420
• Publication Date: 2021-08-06T15:06:33Z
• Authors: Zhicui Liu; Shuai Liu; Yan Zhang; Weihong Zeng; Shujun Wang; Ping Ji; Meng Pan; Cheng Zhu; Ying Wang
• Keywords: Adult; CD4-Positive T-Lymphocytes; Male; 0301 basic medicine; B-Lymphocytes; Adolescent; CD40 Ligand; Cell Communication; Middle Aged; Lymphocyte Activation; Inducible T-Cell Co-Stimulator Protein; Young Adult; 03 medical and health sciences; Focal Adhesion Kinase 2; Immunoglobulin M; Immunoglobulin G; Antibody Formation; Cell Adhesion; Humans; Lupus Erythematosus, Systemic; Female; Cells, Cultured

CD40-CD40L and inducible co-stimulatory molecule (ICOS)-ICOSL ligations are demonstrated to play critical roles in CD4+ T-B interaction for B cell activation differentiation mouse models. Herein, by using a micropipette adhesion assay an vitro coculture system simultaneously, we intended dissect their human IgG/IgM production. With the upregulation of CD40L ICOS expressions on T cells upon TCR/CD28 stimulation vitro, activated exhibited enhanced with autologous at single level when compared resting counterparts. Blockade dramatically damped between whereas less effect blockade was observed. On contrary, led dramatic decrease production were cocultured together induction CD19hi cells. However, displayed attenuation Distinct ICOS-ICOSL also observed lupus erythematosus patients. The CD40L, rather than ICOS, phosphorylation Pyk2 total Our study thus provides evidence that either or pathogenic state function diversely during interactions. While ligation is more likely be engaged adhesion, indispensable signal results indicative segregating costimulation differentiation, which might helpful dissection SLE pathogenesis.