CEL-Seq: Single-Cell RNA-Seq by Multiplexed Linear Amplification

• DOI: 10.1016/j.celrep.2012.08.003
• Publication Date: 2012-08-30T12:17:40Z
• Authors: Tamar Hashimshony; Florian Wagner; Noa Sher; Itai Yanai
• Keywords: RNA-Seq; Transcription; Single-Cell Analysis; Pooling

High-throughput sequencing has allowed for unprecedented detail in gene expression analyses, yet its efficient application to single cells is challenged by the small starting amounts of RNA. We have developed CEL-Seq, a method overcoming this limitation barcoding and pooling samples before linearly amplifying mRNA with use one round vitro transcription. show that CEL-Seq gives more reproducible, linear, sensitive results than PCR-based amplification method. demonstrate power studying early C. elegans embryonic development at single-cell resolution. Differential distribution transcripts between sister seen as two-cell stage embryo, zygotic somatic cell lineages enriched transcription factors. The robust transcriptome quantifications enabled will be useful transcriptomic analyses complex tissues containing populations diverse types.