Poised RNA Polymerase II Changes over Developmental Time and Prepares Genes for Future Expression

• DOI: 10.1016/j.celrep.2012.11.024
• Publication Date: 2012-12-21T04:26:24Z
• Authors: Bjoern Gaertner; Jeff Johnston; Kai Chen; Nina Wallaschek; Ariel Paulson; Alexander S. Garruss; Karin Gaudenz; Bony De Kumar; Robb Krumlauf; Julia Zeitlinger
• Keywords: RNA polymerase II; RNA polymerase III; Developmental Biology

Poised RNA polymerase II (Pol II) is predominantly found at developmental control genes and thought to allow their rapid synchronous induction in response extracellular signals. How the recruitment of poised Pol regulated during development not known. By isolating muscle tissue from Drosophila embryos five stages differentiation, we show that occurs many de novo this makes them permissive for future gene expression. A comparison with other tissues shows these changes are stage specific specific. In contrast, Polycomb group repression specific, combination (the balanced state) marks highly dynamic This suggests temporally held check a tissue-specific fashion. We compare our data findings mammalian embryonic stem cells discuss framework predicting programs on basis chromatin state.