Heterochromatin Reorganization during Early Mouse Development Requires a Single-Stranded Noncoding Transcript

• DOI: 10.1016/j.celrep.2013.08.015
• Publication Date: 2013-09-19T17:54:36Z
• Authors: Miguel Casanova; Michał Pasternak; Fatima El Marjou; Patricia Le Baccon; Aline V. Probst; Geneviève Almouzni
• Keywords: Cell fate determination; Transcription; Genomic Imprinting

The equalization of pericentric heterochromatin from distinct parental origins following fertilization is essential for genome function and development. recent implication noncoding transcripts in this process raises questions regarding the connection between RNA nuclear organization chromatin environments. Our study addresses interrelationship replication transcription two (PHC) domains their reorganization during early embryonic We demonstrate that PHC dispensable its clustering at late two-cell stage. In contrast, using parthenogenetic embryos, we show are independent marks associated with domains. Finally, our discovery only reverse required both development beyond stage challenges current views on organization.