Comprehensive Identification of Host Modulators of HIV-1 Replication using Multiple Orthologous RNAi Reagents

• DOI: 10.1016/j.celrep.2014.09.031
• Publication Date: 2014-10-16T15:45:31Z
• Authors: Jian Zhu; Teresa Davoli; Jill M. Perriera; Christopher R. Chin; Gaurav D. Gaiha; Sinu P. John; Frederic D. Sigio...; Geng Gao; Qikai Xu; Hongjing Qu; Thomas Pertel; Jennifer S. Sims; Jennifer A. Smith; Richard E. Baker; Louise Maranda; Aylwin Ng; Stephen J. Elledge; Abraham L. Brass
• Keywords: Replication

RNAi screens have implicated hundreds of host proteins as HIV-1 dependency factors (HDFs). While informative, these early studies overlap poorly due to false positives and negatives. To ameliorate issues, we combined information from the existing HDF together with new performed multiple orthologous reagents (MORR). In addition being traditionally validated, MORR historical were quantitatively integrated by adaptation an established analysis program, RIGER, for collective interpretation each gene's phenotypic significance. False addressed removal expressed candidates through gene expression filtering, well GESS, which identifies off-target effects. This workflow produced a network genes that modulate replication. We further investigated roles GOLGI49, SEC13, COG in Collectively, MORR-RIGER method minimized caveats screening improved our understanding HIV-1–host cell interactions.