Shugoshin-1 Balances Aurora B Kinase Activity via PP2A to Promote Chromosome Bi-orientation

• DOI: 10.1016/j.celrep.2015.03.052
• Publication Date: 2015-04-17T11:44:28Z
• Authors: Amanda Meppelink; Lilian Kabeche; Martijn J.M. Vromans; Duane A. Compton; Susanne M.A. Lens
• Keywords: Aurora B kinase

Correction of faulty kinetochore-microtubule attachments is essential for faithful chromosome segregation and dictated by the opposing activities Aurora B kinase PP1 PP2A phosphatases. How phosphatase are appropriately balanced less clear. Here, we show that a centromeric pool PP2A-B56 counteracts T-loop phosphorylation recruited to centromeres through Shugoshin-1 (Sgo1). In non-transformed RPE-1 cells, B, Sgo1, enriched on levels diminish as chromosomes establish bi-oriented attachments. Elevating Sgo1 at recruits excess PP2A-B56, this activity, undermining efficient correction attachment errors. Conversely, Sgo1-depleted cells display reduced localization whereas remaining hyperactive due concomitant reduction PP2A-B56. Our data suggest can tune stability recruitment balances activity centromere.